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Immunohistochemical staining of human gall bladder shows cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human gall bladder shows cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human gall bladder shows cytoplasmic and membranous positivity in glandular cells.

Anti-STXBP2 Antibody

HPA015564
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSTXBP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-STXBP2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6813
  • Target name
    STXBP2
  • Target description
    syntaxin binding protein 2
  • Target synonyms
    FHL5, Hunc18b, MUNC18-2, UNC18-2, UNC18B, pp10122, unc-18B, syntaxin-binding protein 2, protein unc-18 homolog B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15833
  • Protein Name
    Syntaxin-binding protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of STXBP2 using anti-STXBP2 antibody (PB9820). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STXBP2 antigen affinity purified polyclonal antibody (Catalog # PB9820) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STXBP2 at approximately 66 kDa. The expected band size for STXBP2 is at 66 kDa.
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