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Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.

Anti-SUGT1 Antibody

HPA069215
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSUGT1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SUGT1 Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10910
  • Target name
    SUGT1
  • Target description
    SGT1 assembly cochaperone of MIS12 kinetochore complex
  • Target synonyms
    SGT1, protein SGT1 homolog, SGT1 homolog, MIS12 kinetochore complex assembly cochaperone, SGT1, suppressor of G2 allele of SKP1, putative 40-6-3 protein, suppressor of G2 allele of SKP1, S. cerevisiae, homolog of
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2Z0
  • Protein Name
    Protein SGT1 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of SUGT1 using anti-SUGT1 antibody (A05411-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUGT1 antigen affinity purified polyclonal antibody (Catalog # A05411-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUGT1 at approximately 36 kDa. The expected band size for SUGT1 is at 41 kDa.
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