Overlay histogram showing HepG2 cells stained with A03500-1 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A03500-1, 1:25 dilution) for 60 min at 37C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) at 1/400 dilution for 40 min at 37C. Isotype control antibody (blue line) was rabbit IgG1 (1microg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.