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Immunohistochemistry analysis in human testis and pancreas tissues using Anti-SUPT5H antibody. Corresponding SUPT5H RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human testis and pancreas tissues using Anti-SUPT5H antibody. Corresponding SUPT5H RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human testis and pancreas tissues using Anti-SUPT5H antibody. Corresponding SUPT5H RNA-seq data are presented for the same tissues.

Anti-SUPT5H-25ul

HPA029273
Atlas Antibodies
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SUPT5H-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    ChIP, ICC, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00267
  • Protein Name
    Transcription elongation factor SPT5
  • Scientific Description
    Rabbit Polyclonal Anti-SUPT5H Antibody against Human suppressor of Ty 5 homolog (S. cerevisiae). Validated for Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry and Western Blot
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

Datasheet

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MSDS

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Figure 1. Western blot analysis of SPT5/SUPT5H using anti-SPT5/SUPT5H antibody (A03426-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPT5/SUPT5H antigen affinity purified polyclonal antibody (Catalog # A03426-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPT5/SUPT5H at approximately 150 kDa. The expected band size for SPT5/SUPT5H is at 121 kDa.
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