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Immunohistochemical staining of human oral mucosa shows strong cytoplasmic and nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human oral mucosa shows strong cytoplasmic and nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human oral mucosa shows strong cytoplasmic and nuclear positivity in squamous epithelial cells.

Anti-SURF2 Antibody

HPA044340
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSURF2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SURF2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6835
  • Target name
    SURF2
  • Target description
    surfeit 2
  • Target synonyms
    SURF-2, surfeit locus protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15527
  • Protein Name
    Surfeit locus protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SURF2 using anti-SURF2 antibody (A14641). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SURF2 antigen affinity purified polyclonal antibody (Catalog # A14641) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SURF2 at approximately 38 kDa. The expected band size for SURF2 is at 30 kDa.
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