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Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

Anti-SVOPL Antibody

HPA018035
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSVOPL
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SVOPL
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID136306
  • Target name
    SVOPL
  • Target description
    SVOP like
  • Target synonyms
    putative transporter SVOPL; SV2 related protein homolog-like
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N434
  • Protein Name
    Putative transporter SVOPL
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: SVOPL antibody at 2microg/ml Lane 1: Mouse liver tissue Lane 2: Rat liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 54, 37 kDa Observed band size: 54 kDa
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Figure 1. Western blot analysis of SVOPL using anti-SVOPL antibody (A16758-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SVOPL antigen affinity purified polyclonal antibody (Catalog # A16758-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SVOPL at approximately 37 kDa. The expected band size for SVOPL is at 54,37 kDa.
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