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Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.

Anti-SYN2 Antibody

HPA037012
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSYN2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SYN2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6854
  • Target name
    SYN2
  • Target description
    synapsin II
  • Target synonyms
    SYNII, synapsin-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA553180(SYN2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
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Figure 1. Western blot analysis of Synapsin II using anti-Synapsin II antibody (PB10100). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synapsin II antigen affinity purified polyclonal antibody (Catalog # PB10100) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Synapsin II at approximately 52, 70 kDa. The expected band size for Synapsin II is at 63 kDa.
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Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with _x000D_ Synapsin 2 Polyclonal Antibody, Unconjugated (bs-3502R) at 1:200 overnight at 4°C, DAB staining.
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Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Synapsin II antibody [GT387] (GTX635883) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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