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Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.

Anti-SYNC Antibody

HPA028311
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSYNC
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SYNC Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID81493
  • Target name
    SYNC
  • Target description
    syncoilin, intermediate filament protein
  • Target synonyms
    SYNC1, SYNCOILIN, syncoilin, intermediate filament protein syncoilin, syncoilin intermediate filament 1, syncoilin-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H7C4
  • Protein Name
    Syncoilin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Syncoilin/SYNC using anti-Syncoilin/SYNC antibody (A06159-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syncoilin/SYNC antigen affinity purified polyclonal antibody (Catalog # A06159-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syncoilin/SYNC at approximately 55 kDa. The expected band size for Syncoilin/SYNC is at 55 kDa.
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