Figure 1. IHC analysis of Syndecan 3/SDC3 using anti-Syndecan 3/SDC3 antibody (PA2133). Syndecan 3/SDC3 was detected in a paraffin-embedded section of Human Placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Syndecan 3/SDC3 Antibody (PA2133) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of Syndecan 3/SDC3 using anti-Syndecan 3/SDC3 antibody (PA2133). Syndecan 3/SDC3 was detected in a paraffin-embedded section of Human Placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Syndecan 3/SDC3 Antibody (PA2133) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Anti-Syndecan 3/SDC3 Antibody
PA2133
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetSdc3
Overview
- SupplierBoster Bio
- Product NameAnti-Syndecan 3/SDC3 Antibody
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- Applications SupplierIHP, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID20970
- Target nameSdc3
- Target descriptionsyndecan 3
- Target synonymsSynd3, mKIAA0468, syn-3, syndecan-3
- HostRabbit
- IsotypeIgG
- Protein IDQ64519
- Protein NameSyndecan-3
- Scientific DescriptionBoster Bio Anti-Syndecan 3/SDC3 Antibody catalog # PA2133. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human, Mouse, Rat
- Reactivity SupplierHuman, Mouse, Rat, Hamster
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
- Yao J, Zhang LL, Huang XM, et al. Pleiotrophin and N-syndecan promote perineural invasion and tumor progression in an orthotopic mouse model of pancreatic cancer. World J Gastroenterol. 2017,23(21):3907-3914. doi: 10.3748/wjg.v23.i21.3907Read this paper
- Yao J, Li WY, Li SG, et al. Midkine promotes perineural invasion in human pancreatic cancer. World J Gastroenterol. 2014,20(11):3018-24. doi: 10.3748/wjg.v20.i11.3018Read this paper
- Yao J, Ma Q, Wang L, et al. Pleiotrophin expression in human pancreatic cancer and its correlation with clinicopathological features, perineural invasion, and prognosis. Dig Dis Sci. 2009,54(4):895-901. doi: 10.1007/s10620-008-0433-5Read this paper
Datasheet
MSDS
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