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Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.

Anti-SYNJ2BP Antibody

HPA000866
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSYNJ2BP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SYNJ2BP
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55333
  • Target name
    SYNJ2BP
  • Target description
    synaptojanin 2 binding protein
  • Target synonyms
    activin receptor interacting protein 5; ARIP2; mitochondrial outer membrane protein 25; OMP25; synaptojanin-2-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP57105
  • Protein Name
    Synaptojanin-2-binding protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Immunoprecipitating SYNJ2BP in Rat kidney tissue Lane 1: Rabbit control IgG instead of CSB-PA023018LA01HU in Rat kidney tissue. For western blotting, a HRP-conjugated light chain specific antibody was used as the secondary antibody (1/50000) Lane 2: CSB-PA023018LA01HU (6microg) + Rat kidney tissue (500microg) Lane 3: Rat kidney tissue (20microg)
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Figure 1. Western blot analysis of SYNJ2BP using anti-SYNJ2BP antibody (A11249-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYNJ2BP antigen affinity purified polyclonal antibody (Catalog # A11249-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYNJ2BP at approximately 16 kDa. The expected band size for SYNJ2BP is at 16 kDa.
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