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Immunohistochemical staining of human kidney shows strong membranous positivity in cells in glomeruli.
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in glomeruli.
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in glomeruli.

Anti-SYNPO Antibody

HPA034631
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSYNPO
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SYNPO Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11346
  • Target name
    SYNPO
  • Target description
    synaptopodin
  • Target synonyms
    SYNPO1, synaptopodin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N3V7
  • Protein Name
    Synaptopodin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Synaptopodin/SYNPO using anti-Synaptopodin/SYNPO antibody (A03154-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human SH-SY5Y whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptopodin/SYNPO antigen affinity purified polyclonal antibody (Catalog # A03154-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Synaptopodin/SYNPO at approximately 99KD. The expected band size for Synaptopodin/SYNPO is at 99KD.
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