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Anti-TAF1 Antibody

144-61170
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetTAF1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-TAF1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6872
  • Target name
    TAF1
  • Target description
    TATA-box binding protein associated factor 1
  • Target synonyms
    BA2R, CCG1, CCGS, DYT3, DYT3/TAF1, KAT4, MRXS33, N-TAF1, NSCL2, OF, P250, TAF(II)250, TAF2A, TAFII-250, TAFII250, XDP, transcription initiation factor TFIID subunit 1, TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa, TBP-associated factor 250 kDa, cell cycle gene 1 protein, cell cycle, G1 phase defect, complementation of cell cycle block, G1-to-S, transcription factor TFIID p250 polypeptide
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21675
  • Protein Name
    Transcription initiation factor TFIID subunit 1
  • Scientific Description
    TAF1 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TAF1 using anti-TAF1 antibody (A02151-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat bain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAF1 antigen affinity purified polyclonal antibody (Catalog # A02151-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAF1 at approximately 250 kDa. The expected band size for TAF1 is at 250 kDa.
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