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Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.

Anti-TAF12 Antibody

HPA008519
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTAF12
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TAF12
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6883
  • Target name
    TAF12
  • Target description
    TATA-box binding protein associated factor 12
  • Target synonyms
    TAF2J, TAFII20, transcription initiation factor TFIID subunit 12, TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa, TAFII-20/TAFII-15, TAFII20/TAFII15, TATA box binding protein (TBP)-associated factor, RNA polymerase II, J, 20kD, transcription initiation factor TFIID 20/15 kDa subunits
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16514
  • Protein Name
    Transcription initiation factor TFIID subunit 12
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of TAF12 using anti-TAF12 antibody (A06944-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAF12 antigen affinity purified polyclonal antibody (Catalog # A06944-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAF12 at approximately 22KD. The expected band size for TAF12 is at 18KD.
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