
Figure 1. Western blot analysis of Tap1 using anti-Tap1 antibody (A01123-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tap1 antigen affinity purified polyclonal antibody (Catalog # A01123-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tap1 at approximately 70 kDa. The expected band size for Tap1 is at 81 kDa.
Anti-Tap1 Antibody Picoband(r)
A01123-2-FITC
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetTap1
Overview
- SupplierBoster Bio
- Product NameAnti-Tap1 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateFITC
- Gene ID21354
- Target nameTap1
- Target descriptiontransporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
- Target synonymsABC17, APT1, Abcb2, Ham-1, Ham1, MTP1, PSF1, RING4, TAP, Tap-1, Y3, antigen peptide transporter 1, ABC transporter, MHC, 1, ATP dependent transport protein family member, ATP-binding cassette sub-family B member 2, ATP-binding cassette transporter, major histocompatibility complex, 1, ATP-binding cassette, sub-family B (MDR/TAP), member 2, histocompatibility antigen modifier 1, peptide supply factor 1, peptide transporter PSF1, peptide transporter TAP1, transporter 1 ATP-binding cassette sub-family B, transporter 1, ABC (ATP binding cassette), transporter associated with antigen processing 1, transporter, ABC, MHC, 1, transporter, ATP-binding cassette, major histocompatibility complex, 1
- HostRabbit
- IsotypeIgG
- Protein IDP21958
- Protein NameAntigen peptide transporter 1
- Scientific DescriptionBoster Bio Anti-Tap1 Antibody Picoband® catalog # A01123-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203

