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Figure 1. Western blot analysis of Tap1 using anti-Tap1 antibody (A01123-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tap1 antigen affinity purified polyclonal antibody (Catalog # A01123-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tap1 at approximately 70 kDa. The expected band size for Tap1 is at 81 kDa.
Figure 1. Western blot analysis of Tap1 using anti-Tap1 antibody (A01123-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tap1 antigen affinity purified polyclonal antibody (Catalog # A01123-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tap1 at approximately 70 kDa. The expected band size for Tap1 is at 81 kDa.
Figure 1. Western blot analysis of Tap1 using anti-Tap1 antibody (A01123-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tap1 antigen affinity purified polyclonal antibody (Catalog # A01123-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tap1 at approximately 70 kDa. The expected band size for Tap1 is at 81 kDa.

Anti-Tap1 Antibody Picoband(r)

A01123-2-FITC
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetTap1
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Tap1 Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    FITC
  • Gene ID21354
  • Target name
    Tap1
  • Target description
    transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
  • Target synonyms
    ABC transporter, MHC, 1; ABC17; Abcb; Abcb2; antigen peptide transporter 1; APT1; ATP dependent transport protein family member; ATP-binding cassette sub-family B member 2; ATP-binding cassette transporter, major histocompatibility complex, 1; ATP-binding cassette, sub-family B (MDR/TAP), member 2; Ham; Ham-; Ham1; Ham-1; histocompatibility antigen modifier 1; MTP; MTP1; peptide supply factor 1; peptide transporter PSF1; peptide transporter TAP1; PSF; PSF1; RI; RING4; T; TAP; Tap-1; transporter 1 ATP-binding cassette sub-family B; transporter 1, ABC (ATP binding cassette); transporter associated with antigen processing 1; transporter, ABC, MHC, 1; transporter, ATP-binding cassette, major histocompatibility complex, 1; Y3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21958
  • Protein Name
    Antigen peptide transporter 1
  • Scientific Description
    Boster Bio Anti-Tap1 Antibody Picoband® catalog # A01123-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203