Anti TAR DNA-Binding Protein 43 (TDP-43), phospho-Ser409/410 mAb (Clone 11-9)

Cosmo Bio USA

Anti TAR DNA-Binding Protein 43 (TDP-43), phospho-Ser409/410 mAb (Clone 11-9)

16

Research Use Only

Anti-TAR DNA-Binding Protein 43 (TDP-43), phospho-Ser409/410 mAb 11-9 was cloned from mice immunized with diphospho-peptide [CMDSKS(p)S(p)GWGM]. 11-9 recognizes human TDP-43 phosphorylated on serines 409 and 410. 11-9 is validated for western blot, ELISA and IHC(f). This antibody is a powerful tool for biochemical and immunohistochemical analyses of TDP-43 proteinopathy. TDP-43, a heterogeneous nuclear ribonucleoprotein, was identified as a component of ubiquitin-positive and tau-negative inclusions observed in cases of frontotemporal lobar degeneration (FTLD-U) and amyotrophic lateral sclerosis (ALS). Immunochemical analyses using antibodies generated against phospho- and non-phosphopeptides of human TDP-43 revealed that abnormally phosphorylated full-length TDP-43 (45 kDa), C-terminal fragments (~25 kDa) and smearing substances are deposited as intracellular inclusions in affected regions of FTLD-U and ALS cases. This antibody is a powerful tool for biochemical and immunohistochemical analyses of neurodegenerative diseases and for evaluation of cellular or animal models of TDP-43 proteinopathy. Cat. No. CAC-TIP-PDT-M01A Product Specifications Important Information on Clone 11-9 Formulation History: Currently shipping (since November 2022) CAC-TIP-PTD-M01A replaces discontinued CAC-TIP-PTD-M01. -M01A (currently shipping; lot nos. 11-9-12-31-1 and newer): Affinity purified in PBS; Concentration: 1.0-1.5 mg/ml. -M01 (lots 11-9-20 and 11-9-21): Ammonium sulfate precipitated; presented in 50% glycerol -MO1 (prior to lot 11-9-20): provided as hybridoma culture supernatant. Recommended dilutions: Western blotting 1/1000-1/3000 Immunohistochemistry 1/3000-1/10000 ELISA 1/1000-1/5000 Other applications not tested Optimal dilutions/concentrations should be determined by end user. References <ol> <ol> Inukai Y, Nonaka T, Arai T, et al. Abnormal phosphorylation of Ser409/410 of TDP-43 in FTLD-U and ALS. FEBS Lett. 582, 2899-2904, 2008 PubMed: 18656473 </ol> </ol>

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