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Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.

Anti-TARBP2 Antibody

HPA061454
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTARBP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TARBP2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6895
  • Target name
    TARBP2
  • Target description
    TARBP2 subunit of RISC loading complex
  • Target synonyms
    LOQS, TRBP, TRBP1, TRBP2, RISC-loading complex subunit TARBP2, TAR (HIV) RNA-binding protein 2, TAR (HIV) RNA-binding protein TRBP1, TAR (HIV-1) RNA binding protein 2, TAR RNA binding protein 2, TARBP2, RISC loading complex RNA binding subunit, trans-activation responsive RNA-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15633
  • Protein Name
    RISC-loading complex subunit TARBP2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of TRBP/TARBP2 using anti-TRBP/TARBP2 antibody (A01680-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRBP/TARBP2 antigen affinity purified polyclonal antibody (Catalog # A01680-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRBP/TARBP2 at approximately 44 kDa. The expected band size for TRBP/TARBP2 is at 39 kDa.
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