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Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Anti-TCP1 Antibody

HPA027337
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTCP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TCP1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6950
  • Target name
    TCP1
  • Target description
    t-complex 1
  • Target synonyms
    CCT-alpha, CCT1, CCTa, D6S230E, IDDPMGS, TCP-1-alpha, T-complex protein 1 subunit alpha, T-complex protein 1, alpha subunit, chaperonin containing T-complex polypeptide 1 subunit 1, t-complex 1 protein, tailless complex polypeptide 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17987
  • Protein Name
    T-complex protein 1 subunit alpha
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of TCP1 using anti-TCP1 antibody (PB9826). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MOLT4 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCP1 antigen affinity purified polyclonal antibody (Catalog # PB9826) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TCP1 at approximately 60 kDa. The expected band size for TCP1 is at 60 kDa.
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