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Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.

Anti-TFDP2 Antibody

HPA070399
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
TargetTFDP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TFDP2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7029
  • Target name
    TFDP2
  • Target description
    transcription factor Dp-2
  • Target synonyms
    DP2; transcription factor Dp-2; transcription factor Dp-2 (E2F dimerization partner 2)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14188
  • Protein Name
    Transcription factor Dp-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TFDP2 using anti-TFDP2 antibody (A06850-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFDP2 antigen affinity purified polyclonal antibody (Catalog # A06850-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFDP2 at approximately 49 kDa. The expected band size for TFDP2 is at 49 kDa.
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