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Immunofluorescent staining of human cell line MCF7 shows localization to vesicles.
Immunofluorescent staining of human cell line MCF7 shows localization to vesicles.
Immunofluorescent staining of human cell line MCF7 shows localization to vesicles.

Anti-THBS3 Antibody

HPA073242
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetTHBS3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-THBS3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7059
  • Target name
    THBS3
  • Target description
    thrombospondin 3
  • Target synonyms
    TSP3, thrombospondin-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49746
  • Protein Name
    Thrombospondin-3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of THBS3 using anti-THBS3 antibody (A11652-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THBS3 antigen affinity purified polyclonal antibody (Catalog # A11652-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for THBS3 at approximately 120 kDa. The expected band size for THBS3 is at 104 kDa.
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