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ProductsBack/Anti-Thrombopoietin [TN1 ]

Anti-Thrombopoietin [TN1 ]

AB00746-1.1-BT
Absolute Antibody
ApplicationsELISA, Neutralisation/Blocking, Other Application
Product group Antibodies
TargetTHPO
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Thrombopoietin [TN1 ]
  • Delivery Days Customer
    9
  • Application Supplier Note
    TN1 can neutralize the biological activity of TPO and can be used to detect TPO in ELISA studies. The Fab fragement of TN1 can also be used to aid the crystallisation of TPO by increasing the area upon which stable crystal contacts can be made (PMID: 11976502).
  • Applications
    ELISA, Neutralisation/Blocking, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    TN1
  • Gene ID7066
  • Target name
    THPO
  • Target description
    thrombopoietin
  • Target synonyms
    c-mpl ligand; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; MKCSF; ML; MPL ligand; MPLLG; myeloproliferative leukemia virus oncogene ligand; prepro-thrombopoietin; THCYT1; thrombopoietin; TPO
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP40225
  • Protein Name
    Thrombopoietin
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Thrombopoietin using anti-Thrombopoietin antibody (A03222-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysate, Lane 2: rat spleen tissue lysate, Lane 3: rat brain tissue lysate, Lane 4: rat kidney tissue lysate, Lane 5: rat thymus tissue lysate, Lane 6: rat PC-12 cell lysate, Lane 7: rat RH35 cell lysate, Lane 8: mouse SP20 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombopoietin antigen affinity purified polyclonal antibody (Catalog # A03222-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thrombopoietin at approximately 38KD. The expected band size for Thrombopoietin is at 38KD.
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