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Immunohistochemical staining of human liver shows strong cytoplasmic positivity(granular pattern) in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity(granular pattern) in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity(granular pattern) in hepatocytes.

Anti-THUMPD3 Antibody

HPA036170
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTHUMPD3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-THUMPD3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25917
  • Target name
    THUMPD3
  • Target description
    THUMP domain 3 tRNA guanosine methyltransferase
  • Target synonyms
    tRNA (guanine(6)-N(2))-methyltransferase THUMP3, THUMP domain containing 3, THUMP domain-containing protein 3, tRNA (guanine(6)-N2)-methyltransferase THUMP3, tRNA(Trp) (guanine(7)-N(2))-methyltransferase THUMP3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BV44
  • Protein Name
    tRNA (guanine(6)-N(2))-methyltransferase THUMP3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of THUMPD3 using anti-THUMPD3 antibody (A15721-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THUMPD3 antigen affinity purified polyclonal antibody (Catalog # A15721-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for THUMPD3 at approximately 68 kDa. The expected band size for THUMPD3 is at 57 kDa.
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