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Anti-TIGIT Antibody, Rabbit Polyclonal

10917-T16
Sino Biological
ApplicationsELISA
Product group Antibodies
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Overview

  • Supplier
    Sino Biological
  • Product Name
    Anti-TIGIT Antibody, Rabbit Polyclonal
  • Delivery Days Customer
    10
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Produced in rabbits immunized with purified, recombinant Human TIGIT/VSTM3 (rh TIGIT/VSTM3; Catalog#10917-H08H; NP_776160.2; Met1-Phe138). TIGIT/VSTM3 specific IgG was purified by Human TIGIT/VSTM3 affinity chromatography.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Tigit using anti-Tigit antibody (A01962-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat plasma lysates, Lane 3: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tigit antigen affinity purified polyclonal antibody (Catalog # A01962-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tigit at approximately 30KD. The expected band size for Tigit is at 30KD.
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FACS analysis of mouse C57Bl/6 splenocytes using GTX01496-07 TIGIT antibody [1G9] (APC). Right panel : co-stained with TIGIT antibody [1G9] (APC) and Mouse CD4 antibody (FITC) Left panel : co-stained with isotype control and Mouse CD4 antibody (FITC) antibody amount : 0.06 microg (5 microl)
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References
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