Anti-TIM-2 [RMT2-14]

Absolute Antibody

Anti-TIM-2 [RMT2-14]

7

This antibody is specific for murine TIM-2, a transmembrane protein that is expressed on activated T cells and preferentially on differentiated Th2 cells. A blockade of TIM-2/TIM-2 ligand interaction results in T cell hyperproliferation and Th2 cytokine p

This antibody has been used in various FACS analyses, for instance, to evaluate the expression of TIM family members on mouse mast cells (Nakae et al, 2007) and to block the H-ferritin binding to TIM-2/L5178Y cells (Kawamoto et al, 2011). This antibody has been used in FACS and immunohistochemistry analyses to show that TIM-2 is expressed on mouse fetal hepatocytes (Watanabe et al, 2007). This antibody has also been used in Western Blots and for blocking experiments in ferritin binding analysis, which suggests that Tim-2 is the H-ferritin receptor on oligodendrocytes (Todorich et al, 2008). In addition, this antibody has been used in collagen-induced arthritis (CIA) models to determine whether TIM-2 contributes to the development of T helper (Th) 1 or Th17 cells and joint inflammation (Kawamoto et al, 2011).

Functional Assay, Flow Cytometry, Western Blot, ImmunoHistoChemistry, Neutralisation/Blocking

FC; WB; IHC; blocking; functional assays

Research Use Only

Monoclonal

RMT2-14

Timd2

T cell immunoglobulin and mucin domain containing 2

AA816106; AU019358; AU019662; C78111; G9D3; T-cell immunoglobulin and mucin domain-containing protein 2; T-cell immunoglobulin mucin receptor 2; T-cell membrane protein 2; Tim; TIM-; Tim2; TIM-2; TIMD-2

Rat

IgG2a

T-cell immunoglobulin and mucin domain-containing protein 2

Mouse

Mouse

This antibody was raised by immunising Sprague Dawley rats with TIM-2-Ig consisting of the extracellular domain (aa 1-230 of mouse TIM-2) and the Fc portion of mouse IgG2a, emulsified in complete Freund's adjuvant and then fusing lymph node cells from the immunized rats with P3U1 myeloma cells.

-20°C,2°C to 8°C

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