Anti-Tim-4 [5G3]

Absolute Antibody

Anti-Tim-4 [5G3]

9

This antibody is specific for murine TIM-4. TIM-4, unlike other TIM family members, is not expressed on T lymphocytes but rather found on antigen-presenting cells such as lymphoid and myeloid dendritic cells and macrophages. TIM-4 has been shown to intera

The original version of this antibody (rat IgG1) specifically bound murine Tim-4 protein, as shown in ELISA and FC assays. The antibody was also used to immunoprecipitate Tim-4 protein from lysates of CHO-Tim-4 as part of WB assays (Rodriguez-Manzanet et al., 2008; PMID: 18354194). This antibody can block apoptotic body (AB) recognition by CD11b+ peritoneal macrophages and partially inhibit AB uptake by peritoneal B-1 cells (Rodriguez-Manzanet et al., 2010; PMID: 20368430). treatment with this antibody alone did not significantly affect tumor burden or survival, but it enhanced the protective effect of anti-PD-1 blockade. The combined treatment resulted in an increase in total CD8+ and CD8+ CD39+ T cells in the peritoneal cavity. RNA sequencing of sorted Tim-4+ macrophages revealed genes differentially expressed between animals treated with anti-PD-1 alone and those treated with both anti-PD-1 and this antibody, indicating that it alters the gene expression profile of Tim-4+ macrophages. Notably, it reduced the expression of genes associated with immunosuppression (Ptgs2, Tgfb2, and Tdo2) while increasing the expression of genes associated with macrophage activation (Stab1 and Mrc1). This antibody was used as part of single-cell RNA sequencing and T-cell receptor sequencing analyses. This antibody was used for FC staining in the form of flow sorting, co-culture experiments, and flow cytometric analysis to investigate various aspects of Tim-4 function, including its role in the sequestration of cytotoxic CD8+ T cells, the impairment of CD8+ T cell proliferation by cavity-resident macrophages, and the assessment of Tim-4-mediated suppression of CD8+ T cells both in vitro and in vivo. For example, it was used for FC staining in the form of annexin V staining to detect phosphatidylserine (PS) exposure on the surface of CD8+ T cells (Chow et al., 2021; PMID: 34115989).

Flow Cytometry, ImmunoPrecipitation, ELISA, Other Application

Research Use Only

Monoclonal

5G3

Timd4

T cell immunoglobulin and mucin domain containing 4

B430010N18Rik; smuckler; spleen, mucin-containing, knockout of lymphotoxin protein; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell immunoglobulin mucin receptor 4; T-cell membrane protein 4; Tim; TIM-; Tim4; TIM-4; TIMD-4

Mouse

IgG2a

T-cell immunoglobulin and mucin domain-containing protein 4

This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Mouse

12352203