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Immunohistochemistry analysis in human tonsil and cerebral cortex tissues using HPA060655 antibody. Corresponding TIMELESS RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human tonsil and cerebral cortex tissues using HPA060655 antibody. Corresponding TIMELESS RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human tonsil and cerebral cortex tissues using HPA060655 antibody. Corresponding TIMELESS RNA-seq data are presented for the same tissues.

Anti-TIMELESS Antibody

HPA060655
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTIMELESS
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TIMELESS
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8914
  • Target name
    TIMELESS
  • Target description
    timeless circadian regulator
  • Target synonyms
    hTIM; protein timeless homolog; TIM; TIM1; timeless circadian clock 1; timeless homolog; Tof1 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UNS1
  • Protein Name
    Protein timeless homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TIMELESS using anti-TIMELESS antibody (A00831). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMELESS antigen affinity purified polyclonal antibody (Catalog # A00831) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMELESS at approximately 160 kDa. The expected band size for TIMELESS is at 138 kDa.
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