Figure 1. Western blot analysis of TIMP1 using anti-TIMP1 antibody (A00561-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMP1 antigen affinity purified polyclonal antibody (Catalog # A00561-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMP1 at approximately 28KD. The expected band size for TIMP1 is at 28KD.
Figure 1. Western blot analysis of TIMP1 using anti-TIMP1 antibody (A00561-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMP1 antigen affinity purified polyclonal antibody (Catalog # A00561-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMP1 at approximately 28KD. The expected band size for TIMP1 is at 28KD.
Anti-TIMP1 Antibody Picoband(r)
A00561-2-10UG
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityMouse, Rat
TargetTimp1
Overview
- SupplierBoster Bio
- Product NameAnti-TIMP1 Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID21857
- Target nameTimp1
- Target descriptiontissue inhibitor of metalloproteinase 1
- Target synonymsCl; Clgi; collagenase inhibitor 16C8 fibroblast; EPA; erythroid-potentiating activity; metalloproteinase inhibitor 1; Timp; TIMP-1; tissue inhibitor of metalloproteinases 1; TPA-induced protein; TPA-S1
- HostRabbit
- IsotypeIgG
- Protein IDP12032
- Protein NameMetalloproteinase inhibitor 1
- Scientific DescriptionBoster Bio Anti-TIMP1 Antibody Picoband® catalog # A00561-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203