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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.

Anti-TJP2 Antibody

HPA001813
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetTJP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TJP2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9414
  • Target name
    TJP2
  • Target description
    tight junction protein 2
  • Target synonyms
    C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1; Friedreich ataxia region gene X104 (tight junction protein ZO-2); PFIC4; tight junction protein ZO-2; X104; ZO2; zona occludens 2; zonula occludens protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UDY2
  • Protein Name
    Tight junction protein ZO-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TJP2/ZO2 using anti-TJP2/ZO2 antibody (A02774-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hek293 whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TJP2/ZO2 antigen affinity purified polyclonal antibody (Catalog # A02774-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TJP2/ZO2 at approximately 150KD. The expected band size for TJP2/ZO2 is at 150KD.
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