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Immunohistochemical staining of human Testis shows strong granular cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human Testis shows strong granular cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human Testis shows strong granular cytoplasmic positivity in Leydig cells.

Anti-TK2 Antibody

HPA041162
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTK2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TK2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7084
  • Target name
    TK2
  • Target description
    thymidine kinase 2
  • Target synonyms
    MTDPS2, MTTK, PEOB3, SCA31, TK2-EXT, thymidine kinase 2, mitochondrial, thymidine kinase 2, 2'-deoxyuridine kinase TK2, deoxycytidine kinase TK2, mitochondrial thymidine kinase, mt-TK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00142
  • Protein Name
    Thymidine kinase 2, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Thymidine Kinase 2/TK2 using anti-Thymidine Kinase 2/TK2 antibody (A03321-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human U-87 MG whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thymidine Kinase 2/TK2 antigen affinity purified polyclonal antibody (Catalog # A03321-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thymidine Kinase 2/TK2 at approximately 25 kDa. The expected band size for Thymidine Kinase 2/TK2 is at 31 kDa.
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