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Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.

Anti-TM9SF3 Antibody

HPA039609
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTM9SF3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TM9SF3
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID56889
  • Target name
    TM9SF3
  • Target description
    transmembrane 9 superfamily member 3
  • Target synonyms
    EP70-P-iso, SMBP, transmembrane 9 superfamily member 3, SM-11044-binding protein, dinucleotide oxidase disulfide thiol exchanger 3 superfamily member 3, endomembrane protein emp70 precursor isolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HD45
  • Protein Name
    Transmembrane 9 superfamily member 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TM9SF3 using anti-TM9SF3 antibody (A12868-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TM9SF3 antigen affinity purified polyclonal antibody (Catalog # A12868-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TM9SF3 at approximately 72 kDa. The expected band size for TM9SF3 is at 68 kDa.
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