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Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.

Anti-TMED5 Antibody

HPA050289
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTMED5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TMED5 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID50999
  • Target name
    TMED5
  • Target description
    transmembrane p24 trafficking protein 5
  • Target synonyms
    CGI-100, p24g2, p28, transmembrane emp24 domain-containing protein 5, p24 family protein gamma-2, p24gamma2, transmembrane emp24 protein transport domain containing 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y3A6
  • Protein Name
    Transmembrane emp24 domain-containing protein 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of TMED5/p28 using anti-TMED5/p28 antibody (A12319-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED5/p28 antigen affinity purified polyclonal antibody (Catalog # A12319-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED5/p28 at approximately 26 kDa. The expected band size for TMED5/p28 is at 26 kDa.
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