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Immunohistochemical staining of human Cerebral cortex shows moderate granular cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human Cerebral cortex shows moderate granular cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human Cerebral cortex shows moderate granular cytoplasmic positivity in neuronal cells.

Anti-TMEFF2 Antibody

HPA015587
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTMEFF2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TMEFF2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23671
  • Target name
    TMEFF2
  • Target description
    transmembrane protein with EGF like and two follistatin like domains 2
  • Target synonyms
    CT120.2, HPP1, TENB2, TPEF, TR, TR-2, tomoregulin-2, cancer/testis antigen family 120, member 2, hyperplastic polyposis protein 1, transmembrane protein TENB2, transmembrane protein with EGF-like and two follistatin-like domains
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UIK5
  • Protein Name
    Tomoregulin-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of TMEFF2 using anti-TMEFF2 antibody (A03846-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEFF2 antigen affinity purified polyclonal antibody (Catalog # A03846-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEFF2 at approximately 41 kDa. The expected band size for TMEFF2 is at 41 kDa.
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