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Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in distal tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in distal tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in distal tubules.

Anti-TMEM107 Antibody

HPA052555
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTMEM107
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TMEM107 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84314
  • Target name
    TMEM107
  • Target description
    transmembrane protein 107
  • Target synonyms
    GRVS638, JBTS29, MKS13, PRO1268, transmembrane protein 107
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6UX40
  • Protein Name
    Transmembrane protein 107
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TMEM107 using anti-TMEM107 antibody (A04966). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM107 antigen affinity purified polyclonal antibody (Catalog # A04966) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM107 at approximately 22KD. The expected band size for TMEM107 is at 16KD.
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