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Immunohistochemical staining of human cerebral cortex shows moderate positivity in glial cells.
Immunohistochemical staining of human cerebral cortex shows moderate positivity in glial cells.
Immunohistochemical staining of human cerebral cortex shows moderate positivity in glial cells.

Anti-TMEM132B Antibody

HPA035661
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTMEM132B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TMEM132B Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID114795
  • Target name
    TMEM132B
  • Target description
    transmembrane protein 132B
  • Target synonyms
    transmembrane protein 132B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14DG7
  • Protein Name
    Transmembrane protein 132B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of TMEM132B using anti-TMEM132B antibody (A16132-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM132B antigen affinity purified polyclonal antibody (Catalog # A16132-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM132B at approximately 95 kDa. The expected band size for TMEM132B is at 95 kDa.
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