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Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Anti-TMEM199 Antibody

HPA027051
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetVMA12
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TMEM199 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID147007
  • Target name
    VMA12
  • Target description
    vacuolar ATPase assembly factor VMA12
  • Target synonyms
    C17orf32, CDG2P, TMEM199, VPH2, vacuolar ATPase assembly protein VMA12, transmembrane protein 199
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N511
  • Protein Name
    Vacuolar ATPase assembly protein VMA12
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TMEM199 using anti-TMEM199 antibody (A14686-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human U2OS whole cell lysates, Lane 8: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM199 antigen affinity purified polyclonal antibody (Catalog # A14686-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM199 at approximately 23KD. The expected band size for TMEM199 is at 23KD.
Product group Antibodies
Boster Bio
Anti-Transmembrane protein 199 TMEM199 Antibody Picoband(r)A14686-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetVMA12
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Atlas Antibodies
PrEST Antigen TMEM199APREST72929
Protein IDQ8N511
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
TMEM199 AntibodyLS-C661085
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetVMA12
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