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Immunofluorescent staining of human cell line A549 shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line A549 shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line A549 shows localization to the Golgi apparatus.

Anti-TMEM5 Antibody

HPA064014
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetRXYLT1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TMEM5 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10329
  • Target name
    RXYLT1
  • Target description
    ribitol xylosyltransferase 1
  • Target synonyms
    HP10481, MDDGA10, TMEM5, ribitol-5-phosphate xylosyltransferase 1, UDP-D-xylose:ribitol-5-phosphate beta1,4-xylosyltransferase, transmembrane protein 5, type II membrane protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2B1
  • Protein Name
    Ribitol-5-phosphate xylosyltransferase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of TMEM5/RXYLT1 using anti-TMEM5/RXYLT1 antibody (A31815-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM5/RXYLT1 antigen affinity purified polyclonal antibody (Catalog # A31815-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM5/RXYLT1 at approximately 56 kDa. The expected band size for TMEM5/RXYLT1 is at 45-51 kDa.
Product group Antibodies
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Anti-TMEM5/RXYLT1 Antibody Picoband(r)A31815-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetRXYLT1
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