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ProductsBack/Anti-TMEM65 (N-term) Antibody

Anti-TMEM65 (N-term) Antibody

102-20469
RayBiotech
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetTMEM65
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-TMEM65 (N-term) Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB27969
  • Gene ID157378
  • Target name
    TMEM65
  • Target description
    transmembrane protein 65
  • Target synonyms
    transmembrane protein 65
  • Host
    Rabbit
  • Protein IDQ6PI78
  • Protein Name
    Transmembrane protein 65
  • Scientific Description
    Rabbit Anti-TMEM65 (N-term) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TMEM65 using anti-TMEM65 antibody (A13650-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM65 antigen affinity purified polyclonal antibody (Catalog # A13650-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM65 at approximately 20 kDa. The expected band size for TMEM65 is at 25 kDa.
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