
Figure 1. Western blot analysis of TMPRSS2 using anti-TMPRSS2 antibody (M00666). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMPRSS2 antigen affinity purified monoclonal antibody (Catalog # M00666) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMPRSS2 at approximately 54 kDa. The expected band size for TMPRSS2 is at 54 kDa.
Anti-TMPRSS2 Rabbit Monoclonal Antibody
M00666
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTMPRSS2
Overview
- SupplierBoster Bio
- Product NameAnti-TMPRSS2 Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID23T34
- Gene ID7113
- Target nameTMPRSS2
- Target descriptiontransmembrane serine protease 2
- Target synonymsepitheliasin; PRSS10; serine protease 10; transmembrane protease serine 2; transmembrane protease, serine 2
- HostRabbit
- IsotypeIgG
- Protein IDO15393
- Protein NameTransmembrane protease serine 2
- Scientific DescriptionBoster Bio Anti-TMPRSS2 Rabbit Monoclonal Antibody catalog # M00666. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203