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Immunohistochemical staining of human smooth muscle shows modrate cytoplamsic positivity in smooth muscle cells.
Immunohistochemical staining of human smooth muscle shows modrate cytoplamsic positivity in smooth muscle cells.
Immunohistochemical staining of human smooth muscle shows modrate cytoplamsic positivity in smooth muscle cells.

Anti-TNFAIP1 Antibody

HPA013333
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTNFAIP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TNFAIP1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7126
  • Target name
    TNFAIP1
  • Target description
    TNF alpha induced protein 1
  • Target synonyms
    B12, B61, BTBD34, EDP1, hBACURD2, BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2, BTB/POZ domain-containing protein TNFAIP1, tumor necrosis factor, alpha induced protein 1, tumor necrosis factor, alpha-induced protein 1 (endothelial)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13829
  • Protein Name
    BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TNFAIP1 using anti-TNFAIP1 antibody (PA1305). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFAIP1 antigen affinity purified polyclonal antibody (Catalog # PA1305) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFAIP1 at approximately 36 kDa. The expected band size for TNFAIP1 is at 25,36 kDa.
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