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Anti-TNFR2 [5E6]

AB03366-1.1-BT
Absolute Antibody
ApplicationsELISA, Neutralisation/Blocking
Product group Antibodies
TargetTNFRSF1B
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-TNFR2 [5E6]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The binding characterization to human TNFR2 was done using ELISA. This antibody can effectively block the binding of TNFR2 with TNF alpha. The binding affinity of the original IgG antibody for human TNFR2 was determined using surface plasmon resonance and found to be Kd= 9.53 X 10^(-10) M. This antibody was also reported to show tumor inhibitory activity in b-HTNFR2 mice (colon cancer cells) (CN112480259).
  • Applications
    ELISA, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5E6
  • Gene ID7133
  • Target name
    TNFRSF1B
  • Target description
    TNF receptor superfamily member 1B
  • Target synonyms
    CD120b; p75; p75 TNF receptor; p75TNFR; p80 TNF-alpha receptor; soluble TNFR1B variant 1; TBPII; TNFBR; TNFR1B; TNFR2; TNF-R2; TNF-R75; TNFR80; TNF-RII; TNF-R-II; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor type II
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP20333
  • Protein Name
    Tumor necrosis factor receptor superfamily member 1B
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNF Receptor II/TNFRSF1B using anti-TNF Receptor II/TNFRSF1B antibody (A01437-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor II/TNFRSF1B antigen affinity purified polyclonal antibody (Catalog # A01437-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor II/TNFRSF1B at approximately 75 kDa. The expected band size for TNF Receptor II/TNFRSF1B is at 75 kDa.
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