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Anti-TNFRSF12A Antibody

144-60182
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetTNFRSF12A
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-TNFRSF12A Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51330
  • Target name
    TNFRSF12A
  • Target description
    TNF receptor superfamily member 12A
  • Target synonyms
    CD266; FGF-inducible 14; fibroblast growth factor-inducible immediate-early response protein 14; FN14; tumor necrosis factor receptor superfamily member 12A; TWEAKR; tweak-receptor; type I transmembrane protein Fn14
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NP84
  • Protein Name
    Tumor necrosis factor receptor superfamily member 12A
  • Scientific Description
    TNFRSF12A Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TWEAKR using anti-TWEAKR antibody (M06261). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HUVEC whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates, Lane 6: mouse 4T1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TWEAKR antigen affinity purified monoclonal antibody (Catalog # M06261) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TWEAKR at approximately 17,20 kDa. The expected band size for TWEAKR is at 17,20 kDa.
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