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Immunohistochemical staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.

Anti-TNFSF12 Antibody

HPA052967
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTNFSF12
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TNFSF12
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8742
  • Target name
    TNFSF12
  • Target description
    TNF superfamily member 12
  • Target synonyms
    APO3L, DR3LG, TNF12, TNLG4A, TWEAK, tumor necrosis factor ligand superfamily member 12, APO3 ligand, APO3/DR3 ligand, TNF-related WEAK inducer of apoptosis, tumor necrosis factor (ligand) superfamily, member 12, tumor necrosis factor ligand 4A, tumor necrosis factor superfamily member 12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43508
  • Protein Name
    Tumor necrosis factor ligand superfamily member 12
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of TWEAK using anti-TWEAK antibody (A02009-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 cell lysate, Lane 2: human PANC-1 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TWEAK antigen affinity purified polyclonal antibody (Catalog # A02009-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TWEAK at approximately 27KD. The expected band size for TWEAK is at 27KD.
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