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Anti-TNFSF18 Antibody

144-66272
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetTNFSF18
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-TNFSF18 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID8995
  • Target name
    TNFSF18
  • Target description
    TNF superfamily member 18
  • Target synonyms
    activation-inducible TNF-related ligand; AITR ligand; AITRL; GITR ligand; GITRL; glucocorticoid-induced TNF-related ligand; glucocorticoid-induced TNFR-related protein ligand; hGITRL; TL6; TNLG2A; tumor necrosis factor (ligand) superfamily, member 18; tumor necrosis factor ligand 2A; tumor necrosis factor ligand superfamily member 18; tumor necrosis factor superfamily member 18
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UNG2
  • Protein Name
    Tumor necrosis factor ligand superfamily member 18
  • Scientific Description
    TNFSF18 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNFSF18 using anti-TNFSF18 antibody (A04408-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TNFSF18 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFSF18 antigen affinity purified polyclonal antibody (Catalog # A04408-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFSF18 at approximately 15KD. The expected band size for TNFSF18 is at 15KD.
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