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Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.

Anti-TNIP1 Antibody

HPA037893
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTNIP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TNIP1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10318
  • Target name
    TNIP1
  • Target description
    TNFAIP3 interacting protein 1
  • Target synonyms
    ABIN-1, NAF1, VAN, nip40-1, TNFAIP3-interacting protein 1, A20-binding inhibitor of NF-kappa-B activation 1, HIV-1 Nef-interacting protein, Nef-associated factor 1 SNP, virion-associated nuclear shuttling protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15025
  • Protein Name
    TNFAIP3-interacting protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of TNIP1 using anti-TNIP1 antibody (A02299-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human T-47D whole cell lysates, Lane 6: rat spleen tissue lysates, Lane 7: rat lung tissue lysates, Lane 8: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNIP1 antigen affinity purified polyclonal antibody (Catalog # A02299-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNIP1 at approximately 80-85KD. The expected band size for TNIP1 is at 72KD.
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