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Immunohistochemical staining of human Skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human Skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human Skeletal muscle shows strong cytoplasmic positivity in myocytes.

Anti-TNNI1 Antibody

HPA028190
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetTNNI1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TNNI1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7135
  • Target name
    TNNI1
  • Target description
    troponin I1, slow skeletal type
  • Target synonyms
    SSTNI; TNN1; troponin I type 1 (skeletal, slow); troponin I, slow skeletal muscle
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19237
  • Protein Name
    Troponin I, slow skeletal muscle
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNNI1 using anti-TNNI1 antibody (A07080-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNNI1 antigen affinity purified polyclonal antibody (Catalog # A07080-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNNI1 at approximately 22 kDa. The expected band size for TNNI1 is at 22 kDa.
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