Figure 1. Western blot analysis of TRAM1 using anti-TRAM1 antibody (A04106). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAM1 antigen affinity purified polyclonal antibody (Catalog # A04106) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAM1 at approximately 55-60 kDa. The expected band size for TRAM1 is at 43 kDa.
Figure 1. Western blot analysis of TRAM1 using anti-TRAM1 antibody (A04106). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAM1 antigen affinity purified polyclonal antibody (Catalog # A04106) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAM1 at approximately 55-60 kDa. The expected band size for TRAM1 is at 43 kDa.
Anti-TRAM1 Antibody Picoband(r)
A04106-FITC
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTRAM1
Overview
- SupplierBoster Bio
- Product NameAnti-TRAM1 Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateFITC
- Gene ID23471
- Target nameTRAM1
- Target descriptiontranslocation associated membrane protein 1
- Target synonymsPNAS8; TRAM; TRAMP; translocating chain-associated membrane protein 1; translocating chain-associating membrane protein; translocation-associating membrane protein 1
- HostRabbit
- IsotypeIgG
- Protein IDQ15629
- Protein NameTranslocating chain-associated membrane protein 1
- Scientific DescriptionBoster Bio Anti-TRAM1 Antibody Picoband® catalog # A04106. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203