Anti-Transferrin receptor [R17 217.1.3]

Absolute Antibody

Anti-Transferrin receptor [R17 217.1.3]

7

This antibody was generated and used in a flow cytometry assay to identify murine hematopoietic progenitor cells expressing the transferrin receptor (Lesley et al., 1984; PMID: 6319033). The original format of this antibody was tested and found to moderately reduce cell growth. A Fab version of this antibody was generated, its binding was tested, and it was compared to its full antibody counterpart. In contrast to the original antibody format, the Fab fragment did not cause receptor down-regulation or accelerated degradation. Both the Fab fragment and the full antibody were successfully used in an IF staining assay (Lesley et al., 1989; PMID: 2653853). The original fotmat of this antibody was demonstrated to undergo receptor-mediated transcytosis across the blood-brain barrier (BBB), as confirmed by capillary depletion studies, and to effectively transport into the mouse brain in vivo (Lee et al., 2000; PMID: 10688622). Injection of this antibody into mice resulted in CD71+ erythroid splenocyte cell depletion (Torow et al., 2015; PMID: 26195040).

Flow Cytometry, Other Application

FC; depletion

Research Use Only

Monoclonal

RI7 217.1.3

Mouse

IgG1

This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

-20°C,2°C to 8°C

41116161