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Anti-Transglutaminase 2 [679-14-E06]

Ab00419-1.1
Absolute Antibody
ApplicationsELISA
Product group Antibodies
TargetTGM2
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Transglutaminase 2 [679-14-E06]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody binds to human Transglutaminase 2, an enzyme that catalyzes the isopeptide bond formation between an amine group and an acyl group at the end of the side chain of a glutamine. Anti-TG2 antibody are found in celiac disease and high levels of transglutaminase are found in Huntingtons and Parkinsons.
  • Applications
    ELISA
  • Applications Supplier
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    679-14-E06
  • Gene ID7052
  • Target name
    TGM2
  • Target description
    transglutaminase 2
  • Target synonyms
    C polypeptide, protein-glutamine-gamma-glutamyltransferase; erythrocyte transglutaminase; G(h); heart G alpha(h); hhG alpha(h); hTG2; isopeptidase TGM2; protein G alpha(h); protein-glutamine deamidase TGM2; protein-glutamine dopaminyltransferase TGM2; protein-glutamine gamma-glutamyltransferase 2; protein-glutamine histaminyltransferase TGM2; protein-glutamine noradrenalinyltransferase TGM2; protein-glutamine serotonyltransferase TGM2; TG(C); TGase C; TGase H; TGase II; TGase-2; TGC; tissue transglutaminase; transglutaminase C; transglutaminase H; transglutaminase II; tTG; tTgase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP21980
  • Protein Name
    Protein-glutamine gamma-glutamyltransferase 2
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Human IgA format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Autoantigen TG2.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TGM2 using anti-TGM2 antibody (A02229). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGM2 antigen affinity purified polyclonal antibody (Catalog # A02229) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGM2 at approximately 80 kDa. The expected band size for TGM2 is at 80 kDa.
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