Anti-Transketolase Mouse mAb

PTM BIO

Anti-Transketolase Mouse mAb

5

Flow Cytometry, Western Blot, ImmunoHistoChemistry Paraffin

WB, IHC-P, FC

Research Use Only

Monoclonal

JMMR-2131

Unconjugated

Mouse

IgG

Transketolase

Transketolase (abbreviated as TK) is an enzyme that is encoded by the TKT gene. It participates in both the pentose phosphate pathway in all organisms and the Calvin cycle of photosynthesis. Transketolase catalyzes two important reactions, which operate in opposite directions in these two pathways. In the first reaction of the non-oxidative pentose phosphate pathway, the cofactor thiamine diphosphate accepts a 2-carbon fragment from a 5-carbon ketose (D- xylulose -5-P), then transfers this fragment to a 5-carbon aldose (D-ribose-5-P) to form a 7-carbon ketose (sedoheptulose-7-P). The abstraction of two carbons from D- xylulose-5-P yields the 3-carbon aldose glyceraldehyde-3-P. In the Calvin cycle, transketolase catalyzes the reverse reaction, the conversion of sedoheptulose-7 -P and glyceraldehyde-3-P to pentoses, the aldose D-ribose- 5-P and the ketose D-xylulose-5-P. In mammals, transketolase connects the pentose phosphate pathway to glycolysis, feeding excess sugar phosphates into the main carbohydrate metabolic pathways. Its presence is necessary for the production of NADPH, especially in tissues actively engaged in biosyntheses, such as fatty acid synthesis by the liver and mammary glands, and for steroid synthesis by the liver and adrenal glands.

Stable for 12 months from date of receipt/reconstitution.

Human

Human

Protein A purified

Store at -20°C. Avoid freeze/thaw cycles.

12352203