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Figure 1. Immunohistochemistry validation of MED12 using Anti-TRAP230 MED12 Antibody (A00828-1). Immunohistochemical analysis of paraffin-embedded human lung cancer. Antibody was diluted at 1:100 (4°C
Figure 1. Immunohistochemistry validation of MED12 using Anti-TRAP230 MED12 Antibody (A00828-1). Immunohistochemical analysis of paraffin-embedded human lung cancer. Antibody was diluted at 1:100 (4°C
Figure 1. Immunohistochemistry validation of MED12 using Anti-TRAP230 MED12 Antibody (A00828-1). Immunohistochemical analysis of paraffin-embedded human lung cancer. Antibody was diluted at 1:100 (4°C

Anti-TRAP230 MED12 Antibody

A00828-1
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetMED12
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-TRAP230 MED12 Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID9968
  • Target name
    MED12
  • Target description
    mediator complex subunit 12
  • Target synonyms
    ARC240, CAGH45, FGS1, HDKR, HOPA, Kto, MED12S, OHDOX, OKS, OPA1, TNRC11, TRAP230, mediator of RNA polymerase II transcription subunit 12, CAG repeat protein 45, Kohtalo homolog, OPA-containing protein, activator-recruited cofactor 240 kDa component, human opposite paired, mediator of RNA polymerase II transcription, subunit 12 homolog, putative mediator subunit 12, thyroid hormone receptor-associated protein complex 230 kDa component, thyroid hormone receptor-associated protein, 230 kDa subunit, trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit), trinucleotide repeat-containing gene 11 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ93074
  • Protein Name
    Mediator of RNA polymerase II transcription subunit 12
  • Scientific Description
    Boster Bio Anti-TRAP230 MED12 Antibody catalog # A00828-1. Tested in IHC, IF, WB, ELISA applications. This antibody reacts with Human, Mouse.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of MED12 using anti-MED12 antibody (A00828-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED12 antigen affinity purified polyclonal antibody (Catalog # A00828-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED12 at approximately 260 kDa. The expected band size for MED12 is at 260 kDa.
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