Figure 1. Western blot analysis of TREML1 using anti-TREML1 antibody (A07682-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREML1 antigen affinity purified polyclonal antibody (Catalog # A07682-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TREML1 at approximately 33KD. The expected band size for TREML1 is at 33KD.
Figure 1. Western blot analysis of TREML1 using anti-TREML1 antibody (A07682-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREML1 antigen affinity purified polyclonal antibody (Catalog # A07682-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TREML1 at approximately 33KD. The expected band size for TREML1 is at 33KD.
Anti-TREML1 Antibody Picoband(r)
A07682-2-FITC
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetTREML1
Overview
- SupplierBoster Bio
- Product NameAnti-TREML1 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateFITC
- Gene ID340205
- Target nameTREML1
- Target descriptiontriggering receptor expressed on myeloid cells like 1
- Target synonymsdJ238O23.3; GLTL1825; PRO3438; TLT1; TLT-1; trem-like transcript 1 protein; triggering receptor expressed on myeloid cells-like protein 1
- HostRabbit
- IsotypeIgG
- Protein IDQ86YW5
- Protein NameTrem-like transcript 1 protein
- Scientific DescriptionBoster Bio Anti-TREML1 Antibody Picoband® catalog # A07682-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203